His2Av (phospho S137) polyclonal antibody
产品名称： His2Av (phospho S137) polyclonal antibody
英文名称： His2Av (phospho S137) polyclonal antibody
产品编号： PAB9920
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phsphopeptide of His2Av.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding S137 of drosophila His2Av.
- Host:
- Rabbit
- Reactivity:
- Fruit fly
- Specificity:
- Reactivity occurs against Drosophila H2AvD pS137 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:4000-1:16000)
Western Blot (1:400-1:1600)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- A deficiency screen for dominant suppressors of telomeric silencing in Drosophila.
Mason JM, Ransom J, Konev AY.Genetics. 2004 Nov;168(3):1353-70.
- 2.
- Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster.
Kimura K, Kodama A, Hayasaka Y, Ohta T.Development. 2004 Apr;131(7):1597-606. Epub 2004 Mar 3.
- 3.
- DNA double-strand break-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis.
Madigan JP, Chotkowski HL, Glaser RL.Nucleic Acids Res. 2002 Sep 1;30(17):3698-705.
- Applications
- Western Blot (Tissue lysate)
- Western blot using His2Av (phospho S137) polyclonal antibody (Cat # PAB9920) shows detection of aband at ~ 15 KDa corresponding to Phospho-His2Av S137 (Lane 2 arrow-head).
Lanes contain either mock-irradiated (Lane 1) or 4000-RAD gamma irradiated (Lane 2) Drosophila melanogaster (3rd instar) larvae brainWC lysate separated on by SDS-PAGE and transferred to nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 500.
Washes and reaction with secondary antibody followed incubation.
Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection.
Personal Communication. Yikang Rong, NIH, CCR, Bethesda, MD.
- Entrez GeneID:
- 43229
- Protein Accession#:
- S08118;P08985;NP_524519
- Gene Name:
- His2Av
- Gene Alias:
- CG5499,H2A.X,H2A.Z,H2AV,H2AX,H2AZ,H2AvD,His2AvD,l(3)05146,l(3)810,l(3)97Dd,l(3)L1602
- Gene Description:
- Histone H2A variant
- Gene Ontology:
- Hyperlink
- Other Designations:
- H2A variant,Histone H2A variant CG5499-PA,histone