PRKCA (phospho S657/Y658) polyclonal antibody
产品名称： PRKCA (phospho S657/Y658) polyclonal antibody
英文名称： PRKCA (phospho S657/Y658) polyclonal antibody
产品编号： PAB7910
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKCA.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S657/Y658 of human PRKCA.
- Host:
- Rabbit
- Reactivity:
- Human, Rabbit, Rat
- Specificity:
- This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition,
- Form:
- Liquid
- Storage Buffer:
- In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:2000)
Immunoprecipitation (5-10 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.
Kawakami Y, Kitaura J, Yao L, McHenry RW, Kawakami Y, Newton AC, Kang S, Kato RM, Leitges M, Rawlings DJ, Kawakami T.Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9470-5. Epub 2003 Jul 24.
- 2.
- The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
Nishizuka Y.Nature. 1988 Aug 25;334(6184):661-5.
- Applications
- Immunoprecipitation
- Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PRKCA antibody. Control (lane 1) and alkaline phosphatase treated (lane 2) precipitates were probed with PRKCA (phospho S657/Y658) polyclonal antibody (Cat # PAB7910). The latter shows no detection of PRKCA after phosphatase treatment.
- Entrez GeneID:
- 5578
- Gene Name:
- PRKCA
- Gene Alias:
- AAG6,MGC129900,MGC129901,PKC-alpha,PKCA,PRKACA
- Gene Description:
- protein kinase C, alpha
- Omim ID:
- 176960
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
- Other Designations:
- aging-associated gene 6,protein kinase C alpha type
- Gene Pathway
- Calcium signaling pathway
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Fc gamma R-mediated phagocytosis
- Focal adhesion
- Gap junction
- Glioma
- GnRH signaling pathway
- Leukocyte transendothelial migration
- Long-term depression
- Long-term potentiation
- MAPK signaling pathway
- Melanogenesis
- Natural killer cell mediated cytotoxicity
- Non-small cell lung cancer
- Pathogenic Escherichia coli infection - EHEC
- Pathways in cancer
- Phosphatidylinositol signaling system
- Tight junction
- Vascular smooth muscle contraction
- VEGF signaling pathway
- Vibrio cholerae infection
- Wnt signaling pathway