Donkey normal serum (10 ml)
Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot
Agrisera IncuBlocker (rabbit antibody)
Kit components: Wash Buffer (Reagent A) and the "all in one" goat anti-rabbit HRP-conjugated secondary antibody in advanced blocking solution (Reagent B).Required: primary antibody of your choice and
AgriseraTMB Diluent (100 ml)
One component ready to use reagent. It contains 2.5 mMol L-1 TMB and Hydrogen Peroxide in a buffer at pH 3.6. It also contains non-toxic stabilizers.